The aim of the present study was to evaluate the antiproliferative potential of phenolic extracts from olive oil

The aim of the present study was to evaluate the antiproliferative potential of phenolic extracts from olive oil, leaves and flowers against MCF-7 breast cancer cells. The Cell proliferation was assessed by tetrazolium salt (MTT) based cell viability protocol. The nuclear morphological changes were investigated. The qualitative and quantitative identification of most important phenolic compounds was determined by electro spray ionization tandem mass spectrometry (ESI-MS/MS).
Using the MTT assay, the results showed that all the extracts (oil, leaves and flowers) exhibited a significant reduction in human breast adenocarcinoma MCF-7 cell viability in a dose dependent manner without affecting the growth of mammary epithelial cell line MCF10.The analysis of western blot and the morphological cell change revealed that all the extracts under study trigger mainly apoptosis in breast cancer cells through the mitochondrial pathway.The analysis of phenolic profile of studied samples showed significant levels of phenolic compounds such as secoiridoids ,simple phenols, and flavonoids. It is suggested that the antiproliferative potential of our extract is mainly due to their unique combination of the quantity and quality of phytochemicals. This study highlight the usefulness of phenolic olive products and by-products as a promising natural source for cancer treatment.