92646517145000 Lab Practical 1

92646517145000
Lab Practical 1: Microscopy
Lab Report
No. Name Student ID Contribution
1 Chew Boon Zhan 20113589 Discussion
2 Omar Ashraf KamalElDinAhmed ElSayadElfar20103111 Introduction, Objectives
3 Khatija Akbar Haji Anver20123664 Results,
4 Hashem Mohammad Sami El Bali 20089930 Materials and Methods
5 Tyra Filiz Koksal20127850 Conclusion
Chew, Omar, Hashem, Khatija, Tyra References
Microscopy
Introduction
“Optical microscope utilized for visually detecting the presence of a microscopic object in a sample and determining the posture of object in sample, so repeated visual observation of object which is quiet an active role due to the fact that it gives a qualitative real data that you see. Not only does it provide an observable data, but also accuracy and reliability of data. Microscope content consist of a microscope stage and an objective lens system with an optical path, provided with a stage and a slide holder including a frame stationary yet a slide carrier movable” (Den Engelse, Flips, Janus Becton & Company, 1993). Compound Microscope is a type of microscopes which contains transmitting lights which can move through cells to observe them and this type of microscope is utilized to observe slides only, whilst the other type of microscope is Stereomicroscope or called Dissection microscope, it observes only the 3D shape of the specimen in petri dish or plate. Stereomicroscope contains incident light which reflects when it hits the surface and utilized familiarly with plates.

The significance of Laboratory lectures is “Understanding well “(Janet, 2007) and enhancing practical skills. Students might achieve and determine how to utilize the mechanism and procedure, so it does not harm you or harm itself. The importance of Microscopes is to “view objects that cannot be seen by the naked eye” (MEFANET, 2014).

Objectives
According to (Stanford, 2012) – laboratory is a place where students investigate, analyze, and reflect.  They test and apply theories and make abstract concepts concrete. Help students to utilize valid apparatus, instruments, measuring tools in an accurate and precise way. Moreover, the differentiation between each instrument and level of accuracy. The main objective is to facilitate students in the understanding of the function, pros and cons of each microscope utilized in the experiment. Furthermore, to observe and determine the colony and indicate each specimen’s morphology.Materials and Method
Materials utilized to prepare and observe Saccharomyces are in the following:
After yeast cultured in a agar plate
Harvested yeast colonies
Prepared a slide dropped with yeast colonies on the top of the slide
Stained with iodine
Covered with slip
Observed under Compound Microscope by utilizing 4X Lens (The Red Color Lens)
Drew yeast colonies shape
Materials utilized to prepare and observe Rhizopus are in the following:
Dropped one drop of Lactophenol cotton blue on a clean glass slide
Cut a strip of Cellophane tape at the length of 4-5 (cm)
Soaked the mid-point of the tape on Rhizopus’s black dots
Sticked the tape on the slide at the mid-point, where it touches the Lactophenol blue cotton
Wrapped both ends of the Cellophane safely
Drew Mold Shape
Method
Both utilized Qualitative method because observation under microscope leads to see an image. Moreover, it is a reliable method.

Results
Description
As Table 1.1 visualizes it indicates the difference between Stereo and Compound Microscopes concerning functions and type of instrument utilized such as Slide, Plate and Petri dish. The Main objective is to determine colony in specimens tested. Colony is present in both Specimens and in different utilize of microscope type. Furthermore, as can be seen on slides and written in materials and method the stain added to indicate the correct observation. In Yeast utilized Iodine, whilst Mold Lactophenol Blue. Two different microscopes utilized to have an overview about both specimens in 2D and 3D shapes. Regarding Drawing and Labeling part, it observes the unknown to be known by labeling procedure to have a better understanding and consideration to the type and its shape remains and becomes non-volatile.
Table 1.1 Observation of Colony in Specimens by using different microscopes
Name Of Specimen Type Of Fungus Observation Of Colony
Microscope Drawing and Labeling
Stereo/Dissection Microscope Compound Microscope Stereo/Dissection Microscope Compound Microscope
Plate /Petri Dish Slide Plate /Petri Dish Slide
Yeast Saccharomyces -127009144000 1333511112500
Mold Rhizopus-222259080500 323859207500

609600889000

Figure 1.1 Mold (Rhizopus) on plate

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Figure 1.2 Mold (Rhizopus) on Slide

78105017208500
Figure 1.3 Yeast (Saccharomyces) on plate

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Figure 1.4 Yeast (Saccharomyces) on Slide
References
Den Engelse, P., Flipse, A., JanusBecton, W., & Company, D. (1993). US4833382A – Method and apparatus for use in microscope investigations – Google Patents. Retrieved from https://patents.google.com/patent/US4833382A/enJanet, D. (2007). How important are labs for learning science?. Retrieved from http://scienceblogs.com/ethicsandscience/2007/03/28/how-important-are-labs-for-lea/Stanford, T. (2012). Laboratory Teaching Guidelines | Teaching Commons. Retrieved from https://teachingcommons.stanford.edu/resources/teaching-resources/teaching-strategies/laboratory-teaching-guidelinesMEFANET, C. (2014). Microscopic techniques – WikiLectures. Retrieved from https://www.wikilectures.eu/w/Microscopic_techniques